![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![2003/04 Alessandro Bogliolo Primer design. ©2003/04 Alessandro Bogliolo Outline 1.Polymerase Chain Reaction 2.Primer design. - ppt download 2003/04 Alessandro Bogliolo Primer design. ©2003/04 Alessandro Bogliolo Outline 1.Polymerase Chain Reaction 2.Primer design. - ppt download](https://images.slideplayer.com/19/5780661/slides/slide_17.jpg)
2003/04 Alessandro Bogliolo Primer design. ©2003/04 Alessandro Bogliolo Outline 1.Polymerase Chain Reaction 2.Primer design. - ppt download
![Versatility of different melting temperature (Tm) calculator software for robust PCR and real-time PCR oligonucleotide design: A practical guide | Mohammad Javad Najafpanah - Academia.edu Versatility of different melting temperature (Tm) calculator software for robust PCR and real-time PCR oligonucleotide design: A practical guide | Mohammad Javad Najafpanah - Academia.edu](https://0.academia-photos.com/attachment_thumbnails/49507878/mini_magick20190131-11629-1awhg5m.png?1548951825)